Journal: New biotechnology

Article Title: Clinically compatible advances in blood-derived endothelial progenitor cell isolation and reprogramming for translational applications.

doi: 10.1016/j.nbt.2021.02.001

Figure Lengend Snippet: Fig. 1. EPC morphology and gene expression. (A) Appearance of a freshly isolated EPC colony at passage 0 on day 9 of culture. (B) Appearance of EPCs in culture at passage 2, (C–F) EPCs express the endothelial cell specific markers CD31 (C, red, DAPI blue) and CD144 (D, green, DAPI blue) and pluripotency associated markers NANOG (E, red) and REX-1 (F, red, DAPI blue). Images A-B at 50X magnification, images C, D, E, F at 100X magnification.

Article Snippet: Primary EPCs were stained using specific antibodies against CD31 (Cat. no. BBA7, R&D Systems, Abingdon, UK), CD144 (Cat. no. MAB9381, R&D Systems) and REX1 (Cat. no. AF3598, R&D Systems), or by using ULEX (Cat. no. RL-1062-2, Vector Laboratories) or DAPI (Cat. no. H-1200-10, Vector Laboratories) at the dilution recommended by the manufacturer.

Techniques: Gene Expression, Isolation

Journal: JCI insight

Article Title: A collagen IV-derived peptide disrupts α5β1 integrin and potentiates Ang2/Tie2 signaling.

doi: 10.1172/jci.insight.122043

Figure Lengend Snippet: Figure 5. AXT107 influences cell-cell junctions through Tie2-associated mechanisms. (A) Representative Western blot images of lysates from MECs treated with 200 ng/ml Ang2 and various concentrations of AXT107 targeting phospho-MLC2, with GAPDH as a loading control. (B) Densitometric analysis of images in A (n = 2 or 3). ***P < 0.001 by 1-way ANOVA relative to Ang2-alone control. (C) Representative immunofluorescence images of HUVEC mono- layers treated with 200 ng/ml Ang2, negative control, or Tie2 siRNA, and DMSO or AXT107 that have been stained with DAPI (blue) and antibodies against VE-cadherin (green) and F-actin (red). Scale bars: 25 μm. (D) Ratios of actual perimeters to minimal perimeters for images related to C. Ratios presented as averages from at least 3 cells from 3 different wells and 2 separate experiments (total of 5 values). ***P < 0.001 by 2-tailed Student’s t test.

Article Snippet: The cells were then blocked in blocking buffer (5% normal goat serum, 0.3% Triton X-100 in DPBS containing Ca2+ and Mg2+) and stained for 16 hours with primary antibodies against phospho-Tie2 (Y992) (R&D Systems, catalog AF2720), total Tie2 (Genetex, catalog GTX107505), VE-cadherin (Cell Signaling Technology, catalog 2500), or ZO-1 (ThermoFisher Scientific, catalog 33-9100) diluted 1:150 in antibody dilution buffer (1% BSA; 0.3% Triton X-100 in DPBS containing Ca2+ and Mg2+).

Techniques: Western Blot, Control, Immunofluorescence, Negative Control, Staining